Bacterial resistance reversion of Crateva adansonii (Capparidaceae) on bacterial strains isolated from chronic wounds diagnosed in the district of Ouinhi, Benin

Authors

  • Hervé Kouessivi Janvier BOKOSSA Université d'Abomey-Calavi
  • Parfait DJOSSOU Centre Interfacultaire de Formation et de Recherche en Environnement pour le Développement Durable
  • Théodore SOUSSIA Institut national médico-sanitaire, Université d’Abomey-Calavi, République du Bénin

DOI:

https://doi.org/10.4314/rasp.v6i3.8

Keywords:

Key words: Bacterial resistance, Chronic wound, Antibiotic, Crateva adansonii, Benin

Abstract

Antibiotic resistance has become a significant public health problem worldwide, making treating bacterial infections more difficult and increasing the risk of serious complications. New molecules are, therefore, urgently needed to control the surge in antibiotic resistance. The present study aimed to investigate the reversion of bacterial resistance to the aqueous mixture of Crateva adansonii in combination with conventional antibiotics (ATB) in bacterial strains isolated from chronic wounds diagnosed in the Ouinhi population in Benin. To achieve this, strains were tested for susceptibility to conventional antibiotics using the solid-state antibiotic susceptibility test method, followed by the determination of the Minimum Inhibitory Concentrations (MICs) of both conventional antibiotics and the aqueous mixture of C. adansonii using the colourimetric plate microdilution method. Finally, bacterial resistance reversion was investigated based on the fractional inhibitory concentration (FIC), defined by the ratio of FIC (Extract + ATB) to FIC ATB. The results of the various tests show that the reversion of bacterial resistance resulted in a synergistic action of the binary Extract-ATB mixture with Fractional Inhibitory Concentration (FIC) ˂ 0.5 in contact with the various bacterial strains tested for all associated conventional antibiotics (amoxicillin, ciprofloxacin, trimethoprim-sulfamethoxazole, gentamicin and ampicillin). The MIC values of the mixture obtained were in the range of 3.0516.10-5 mg.mL-1 ≤ MIC (Extract-ATB) ≤ 0.5 mg.mL-1. C. adansonii could well be one of the solutions to the problem of treating chronic wounds in Benin and worldwide.

Author Biographies

  • Parfait DJOSSOU, Centre Interfacultaire de Formation et de Recherche en Environnement pour le Développement Durable

    Doctor in Environment and Public Health. He works on issues of public health and neglected tropical diseases. He is a researcher at the University of Abomey-Calavi.

  • Théodore SOUSSIA, Institut national médico-sanitaire, Université d’Abomey-Calavi, République du Bénin

    Dr Théodore Soussia is a Research Lecturer at the Institut national médico-sanitaire, University of Abomey-Calavi, Republic of Benin. He is interested in public health issues within this institute.

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Published

2025-01-01

Issue

Vol. 6 No. 3 (2024): CINI 2024

Section

CINI2024

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Copyright (c) 2024 Hervé Kouessivi Janvier BOKOSSA, Parfait DJOSSOU, Théodore SOUSSIA

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How to Cite

Bacterial resistance reversion of Crateva adansonii (Capparidaceae) on bacterial strains isolated from chronic wounds diagnosed in the district of Ouinhi, Benin. (2025). Revue Africaine Des Sciences Sociales Et De La Santé Publique, 6(3), 105-120. https://doi.org/10.4314/rasp.v6i3.8